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The Bioptechs, Inc. Delta T
Culture Dish System is designed to simulate host conditions
on the stage of your microscope and provide an optimal optical
environment for microscopy. This two-step system allows
you to plate your cells and observe them without having
to transfer them to another structure. The system components
are, the Controller, Stage
Adapter, and Dishes. Accessories for Tissue
Slice, Brain
Slice and other specimens are available.
An intelligent feedback loop passes an electrical current
through a thin film coating on the underside of the glass
substrate on which the cells are grown. Heat is applied
directly to the cells without the inefficiencies associated
with peripheral heating by traditional culture dish warmers.
Bioptechs exclusively offers opaque culture dishes which
eliminates the unwanted ambient light background for fluorescence
imaging.
The controller features a real-time temperature display
and fast learning curve to compensate for cooling due to
surface evaporation while responding to temperature changes
due to perfusion. There is also an alarmed protection circuit
to safeguard the cells and an internal reference for the
user adjustable calibration. The standard controller has
a temperature range of ambient to 50 degrees C, extended
ranges are available upon request.
Delta T dishes have 35mm O.D. and a 23mm central aperture,
the peripheral region of the dish is tapered to reduce the
dead-volume and the height of the dish is 6mm to allow better
access for micro-injection and micromanipulation. The dishes
are a hybrid of polystyrene plastic and Dasog 243 glass.
The outer structure of the dish is available in opaque black
or clear and come with a clear 0.5mm or no.1.5 glass coverslip
bottom bonded to it. The dishes are also available
in a plain glass unheated version.
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