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How it works:
Cells are plated on a 40mm
coverslip and placed into a special FCS2 chamber. This provides
a perfuseable laminar flow optical chamber with user modifiable
flow characteristics. The upper glass element (Microaqueduct
slide) is then used to remove heat from the specimen cavity
to be absorbed in the cooled fluid being circulated in the cavity
formed by the addition of an O-ring sealed window adapter. Gravity
or an electric pump is then used to create a flow of chilled
fluid through the heat exchange cavity in the chamber. The cells
remain safely enclosed in a separate optical enclosure. If more
thermal transfer is necessary, tubing can be installed into
the FCS2 base.
Note: If you are using high N.A. objectives at below ambient
temperatures it will be necessary to cool and thermally isolate
the objective from the scope. The optional thermal isolator
will prevent condensation from forming on the lower element
of the objective. A gas port is provided to purge the adapter
with dry nitrogen. An anti-reflection coated window optimized
for 340-700nm seals the bottom of the adapter.
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