Lambda Micro-Perfusion Pump
When imaging cells on a coverslip in a chamber where liquid is confined, any changes in flow rate can translate to changes in pressure. This causes the coverslip to exhibit diaphragm-like behavior. Given the narrow depth of field of microscope objectives, this coverslip flexing causes the cells to go out of focus. To eliminate this problem at the lower flow rates, and significantly reduce it at higher flow rates, Bioptechs recommends the Lambda pump for use with all its micro-observation systems.
The Lambda Pump is a miniature, single or dual, channel full-featured peristaltic pump designed specifically for low flow rates. Unlike most peristaltic pumps that are driven by stepper motors, the Lambda pump is driven by a tachometer regulated, multi-stage DC gear motor. This assures a smooth analog rotation of the roller spindle, free of instantaneous steps. It is regulated by either the internal control circuitry adjustable from 2-180 ml/hr or it can be interfaced with a computer through a DIO port. The pump comes with an external 9 volt AC adapter and also contains an internal 9 volt battery which can function as the primary power supply if needed.
The pump includes a single .093" I.D. tube, and a dual tube that has two .062" I.D. tubes for use as a dual channel pump. Although other tubing sizes are available, these two sizes are best suited for imaging applications. The pump tubes are made of silicone rubber and are terminated with a 1/16" tubing barb. The base of the pump is threaded for easy mounting to a stand or fixture near the microscope.
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