PRIME TECH PMM-150FU PIEZO IMPACT DRIVE

 

·         High-resolution one dimensional patented linear piezo drive.

 

·         Single and multiple pulse modes.

 

·         Compatible with many 3-axis micromanipulators

 

·         Facilitates penetration of tough oolemma membranes maximising oocyte survival

 

 

The Prime Tech PMM-150FU uses the piezoelectric effect to generate linear vibrations of a micropipette holder in contact with the piezo element.  The finely controlled ‘stabbing’ motion of the micropipette assists penetration in otherwise difficult microinjection applications, reducing distortion of the oocyte membrane.  This limits trauma to the oocyte and ensures best possible survival rate following injection.

 

Since Prime Tech introduced its first piezo impact micromanipulation system in 1990 the company has been at forefront in producing and developing systems involving this technique.

 

The PMM has now become the system of choice for applications such as mouse nuclear transfer, ICSI and blastocyst injection. In operation the drive unit itself (MB-U) is mounted to a suitable 3-axis micromanipulator.  The standard AD-NE adaptor permits the unit to be mounted to most Narishige illumination support mounting hydraulic micromanipulators.  Other adaptors are available as accessories. 

 

 

 

MB-U Drive Unit

 

Controls on the OP-15 operation box enable two sets of speed and intensity conditions to be selected including single and multiple pulse modes.  The user can toggle between these two condition sets from the operation box or using the footswitch provided.

 

 

 

 

 

Mounting adaptors for MB-U

 

 

 

 

 

 

 

 

AD-BXW              Option.  For Sutter’s XenoWorks micromanipulator.

 

NOTE:                    For mounting the MB-U to Eppendorf’s TransferMan NK2 or PatchMan NP2 an

                                Eppendorf X-Y Extension plate is required.  This is Eppendorf part no.

                                5181 236.002

 

 

SPECIFICATIONS             PMM-150FU

 

CONTROLLER

Single output (controls 1 MB-U unit)

PIEZO IMPACT DRIVE UNIT

(MB-U)

Movement range: approx. + 5mm

Maximum speed: 0.08mm/s

Resolution: approx. 0.1μm

OPERATION BOX

Adjustment dials: 2 sets

          (Speed 16 levels, intensity 16 levels)

Indicator lamps: Condition A (green)

                           Condition B (yellow)

Selection Switch: A – B

Movement keys: Left and/or Right

FOOTSWITCH

Left pedal: moves to left only

Right pedal: Selects condition A or B

DIMENSIONS & WEIGHT

CONTROLLER

OPERATION BOX

 

«MB-U» / IMPACT UNIT

«MB-U» SLIDE BASE UNIT

FOOTSWITCH

 

240(W) x 340(D) x 140(H) mm     Weight approx. 5.0kg

100(W) x 160(D) x 60(H) mm       Weight approx. 530g (including

                                                                                           cable)

125g (including cable)

75g

145(W) x 140(D) x 38(H)mm    Weight approx. 820g (including

                                                                                        cable)

     

OPERATING TEMP.

5 ~ 40°C (Avoid high humidity)

POWER

AC100 ~ 240v, 50/60Hz, 60VA

ACCESSORIES

Power cord, Spare fuse

 

 

 

Microinjection using the PMM

 

·       Precise manual operation

 

·       Excellent sensitivity controllability and reliability

 

·       Compact

 

 

 

 

 

 

 

 

 

 

 

 


The PrimeTech HDJ-M3 microinjector is recommended for use with the PMM-150FU ensuring maximum sensitivity and optimised performance during micromanipulation.  The HDJ-M3 allows injection of spermatozoa, DNA and/or other solutions into somatic cells, unfertilised eggs and embryos.

 

 

Specifications

 

Accessories included

IH-3 injection holder 4mm o.d. for use with 1mm o.d. capillaries

Teflon tube 1m (2mm o.d)

Teflon Luer connector (2)

Silicon tube 6mm (20)

Dimensions (mm)

70 x 182.5 x 90 (WxDxH)

 

Weight

880g

Syringe capacity

9ml

Volume per turn

3.9μl

Screw stroke

20mm

 

When the HDJ-M3 is combined with the PMM-150FU the system is known as the PMM-150HJ.  The PMM-150FU may also be used in conjunction with other microinjectors such as Eppendorf’s CellTram or Narishige syringe-type injectors IM-9B or IM-6.

 

ORDERING INFORMATION

 

 

PMM-150FU

Piezo Impact Drive System comprising:

MB-U piezo impact drive unit (includes IH-3 injection unit)

PMAS-CTI50 Controller

OP-15 Operation box

OP-14 Footswitch

AD-NE mounting adaptor (Narishige type)

Operating manual

PMM-150HJ

Piezo Impact Drive System as above but including HDJ-M3 manual injector

ADAPTORS

AD-NE

AD-NSL

AD-LBK

AD-BXW

 

(Standard) Narishige type

for Narishige stage mount

for Leica.  Universal joint type

for Sutter Xenoworks

INJECTOR

HDJ-M3

 

Manual microinjector including IH-3 injector holder, tubing, connectors and operating manual

PARTS

IH-3

 

OTI-SS1

OCT-SF10

 

 

 

OCT-EPC

 

 

Injection Holder, for 1mm o.d. glass capillary and 2mm o.d. Teflon tube.

Spacer set for IH-3 including spacer (1) and silicone tube (2 x 50mm).

Tubing set for IH-3 (1m Teflon tube 2mm o.d. and Teflon Luer connectors 2).  Supplied as standard with HDJ-M3.

Note: If you will use the Xenoworks microinjector this tubing set will be needed.

Tubing set for Eppendorf CellTram (1m Teflon tube 2mm o.d., Teflon Luer connector and SUS connector for CellTram)

 

 

  • If you use a Narishige injector the 2mm o.d. Narishige tubing is compatible with the IH-3 and so no additional tubing set is necessary.

 

  • Before purchasing a PMM System please let us know the make and model 3-dimensional micromanipulator that you will use.

 

 

 

 

Intracel Ltd

Unit 4 Station Road
Shepreth, Royston
Herts, SG8 6PZ
Tel: 01763 262680 Fax: 01763 262676

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